![]() As the sap is used, oxygen in the heartwood is depleted (creating anaerobic conditions), methane is produced, the pH of the sap is increased (pH 6 in healthy trees to pH 7 to 8 in wetwood), and a high pressure develops in the wood (60 psi in affected trees vs. ![]() The bacteria, including species of Clostridium, Bacillus, Enterobacter, Klebsiella, and Pseudomonas, grow within the tree using the sap as a nutrient source.
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